Occludin在胃癌耐药细胞SGC7901中mRNA水平

Incressing of occludin in SGC7901/VCR may be involved in gastric cancer MDR

肿瘤多药耐药现象是肿瘤化疗研究的热点。且前已经发现的多药耐药机制主要涉及Pgp、MRP、LRP、GSH/GST、TopoⅡ和多种凋亡相关基因的变化。但这些还不能圆满解释肿瘤多药耐药现象。本所制备的胃癌耐药细胞系SGC7901/VCR细胞形态与亲本细胞SGC7901相比,细胞扁平,排列紧密,细胞间紧密连接非常突出。RNA点杂交结果初步表明参与紧密连接的重要分子Occludin在胃癌耐药细胞SGC7901/VCR中表达显著高于其亲本细胞SCG7901,而SGC7901又明显高于fas基因转导的耐药细胞SGC7901/VCR,提示Occludin可能通过调节细胞紧密连接处通透性参与肿瘤多药耐性(MDR)产生。这可望为探讨胃癌耐药新机制奠定一定的基础。

Tumor multidrug resistance(MDR) is a hotsopt in research on chemotherapy. Pgp、MRP、LRP、GSH/GST、Topo Ⅱ and some genes related apoptosis have been confirmed to be responsible for tumor MDR. However, all of these could not fully explain how tumor cells develop MDR. Our department established a MDR gastric cancer cell subline SGC 7901/VCR which arrange tightly, contain more, compared with control cell SGC7901. Dot blot showed higher expression of an important tight junction protein-occludin in SGC7901/VCR than SGC7901. This suggested tight junction may be involvd in gastric cancer MDR by upregulation of occludin.