原发性肝癌血管形成的免疫组化和形态学研究

Study on angiogenesis of primary hepatocellular carcinoma

目的研究原发性肝癌血管形成及血管起源。方法用病理及CD34、UEA-1、Collagen Ⅳ免疫组化的方法对21例肝癌及癌旁组织中血管形成进行原位检测和观察。结果阳性反应可见血管星棕色或棕黄色条带。癌旁组织血窦内皮细胞CD34及UEA-1染色阴性,炎症组织内的血管染色阳性。部分肿瘤血管与癌旁组织血管不相连;部分肿瘤血管与癌旁组织血管相连。Collagen Ⅳ在肝癌组织中有较强的表达,癌旁组织中表达很弱,但在门脉区炎症组织内表达可较强。结论CD34、UEA-1、Collagen Ⅳ染色与肿瘤血管形成有关,部分肝癌血管可能起源于毛细血管化的癌旁肝血窦。

Objective This study was to evaluate the angiogenesis of primary hepatocellular carcinoma (PHC), Methods: The angiogenesis in the 21 patients with PHC were studied by immunohistochemistry of CD34、Ulex europaeua agglutinin 1 (UEA-1) and Collagen Ⅳ. Results: The positive staining of CD34 and UEA-1 could not be seen neither at the sinusoidal endothelial cells of liver cirrhosis nor chronic liver hepatitis, but they could be seen at the vessels of inflamed tissues. Meanwhile they were marked stained at the endothelial cells of PHC. The expression of type Ⅳ collagen was mild in normal livers, moderate in inflamed tissue adjacent to PHC, and rather severe in PHC. Some sinusoidal endothelial cells around the HCC were in continuity with the HCC blood vessels. Conclusion: These findings suggested that the expression of CD34、UEA-1、and Collagen Ⅳ were associated with PHC angiogenesis and the PHC vessels might be derived from the capillarized sinusoidal endothelial cells in the surrounding liver.