体外培养的破骨细胞细胞化学测量观察

Quantitative cytochemical investigation of cultured osteoclasts in vitro

本文采用Ｓｐｒａｑｕｅ－Ｄａｗｌｅｙ大鼠体外破骨细胞培养及新鲜长骨组织破骨细胞印片方法研究两种材料中破骨细胞细胞化学的异同，检测了酸性磷酸酶（ＡＣＰ）抗酒石酸酸性磷酸酶（ＴＲＡＣＰ）、β－酸性半乳糖苷酶、β－葡萄糖醛酸酶、乳酸脱氢酶、琥珀酸脱氢酶、ＮＡＤＨ和ＮＡＤＰＨ脱氢酶，并使用Ｚｅｉｓｓ氏细胞扫描显微镜连接ＣＤ／ＭＤ２０电脑图像分析系统，定量检测了以上酶的活性。结果表明，大鼠体外培养和新鲜组织印片的破骨细胞含有丰富的多种溶酶体酶和厌氧脱氢酶（线粒体酶），新鲜组织印片的破骨细胞大多数脱氢酶的活性高于体外培养的破骨细胞。

Osteoclasts are multinuclear cells formed by the fusion of marrow monocyte-granulocyte lineage. In isolated osteoclast culture system, osteoclasts have a very short life span and osteoclast precursor cells are unable to form multinuclear osteoclasts in culture. It appears that they are somewhat different in their cellular metabolism after osteoclasts are isolated from bone, apart from the changes in the microenvironment.Therefore, the aim of this study was to address the cytochemical differences between the cultured and 'in vivo' osteoclasts using quatitative cytochemistry. The results have indicated that osteoclasts from cultured and imprinted preperations express ACP, TRACP, β-acid galactosidase, β-glucuronidase,lactate dehydrogenase, succinate dehydrogenase, NADH dehydrogenase, and NADPH dehydrogenase.Both cultured and imprinted 'in vivo' osteoclasts contain an abundance of various lysosomal and anaerobic dehydrogenase enzymes for bone resorption but activities of most dehydrogenase in cultured osteoclasts are lower than in imprinted osteoclasts.