黔岭藿对体外培养的破骨细胞作用的研究

Effect of Chinese herb epimedium leptorrhizm stearn on osteoclastic bone resorption

从新生兔四肢长骨中分离的破骨细胞与牛骨片在体外培养后，加入不同浓度黔岭霍注射液再培养，并设立对照，用倒置相差显微镜观察黔岭霍对破骨细胞形成骨吸收陷窝的影响。结果显示黔岭著３个浓度组（０．１５ｍｇ／ｍｌ、１．５ｍｇ／ｍｌ、１５ｍｇ／ｍｌ）与对照组比较均能抑制破骨细胞在骨片上形成吸收陷窝的数量，增加药物浓度抑制作用亦趋增强（Ｐ＜０．０５）。

Osteoclasts were isolated from the long bones of newborn rabbits and cultured on bovine slices in vitro. Each bone slice was placed in a wall of a 24-well culture plate containing various concentrations of Chinese herb epimedium leptorrhizm Stearn in medium 199 or in control medium. Under phase-contrast microscope, the effect of epimedium leptorrhizm Stearn on bone resorption lacunae generated by osteolasts was observed. The results show that all of the concentrations of epimedium leptorrhizm Stearn (0.15mg/ml, 1.5mg/ml, 15mg/ml) have significant inhibitory effect (P<0. 05),which is increased with concentration of drugs.