幽门螺杆菌的长丝体变异及返祖

Threadlike Bacteria Variation of Helicobacter pylori and its reversion

我们采用改良Hp培养基(HBSV培养基)对Hp进行自然诱导,发现Hp球形变异体在含有适量蛋黄和血清的培养中容易返祖及传代,部分培养超过5天的菌落中有长丝体形成,或在球变体两侧形成长短不等的菌丝。长丝体变异的菌落与一般无异,但形态多较大,长丝体约为典型菌的3～10倍以上,菌体弯曲不规则或缠绕,粗细不均,胞壁缺陷,但可在普通渗透压的平板上生长。长丝体不稳定,接种于Hp或HBSV培养基返祖成典型的“S”形Hp。本研究提出这样的假说:Hp变异由典型“S”形→球变→长丝体和巨球体变异→返祖成典型Hp,Hp的这种变异可能存其传染和感染复发中起重要作用。

Improved culture media, HBSV culture media, were used to induce the variation of Helicobacter pylori (H. priori). We found that coecoid H. pylori is easy propagation and reversion to typical form on this medium that is H. pylori culture medium added with 0.5％ vitellus and 2％ vitulary serum. Threadlike bacteria variation was found on some HBSV media, which cultured over 5 days, and the colonies were not special in shape but bigger than normal. The threadlike H. pylori is 3 to 10 times or over bigger than typical H. pylori, with the body threadlike flexuosity and aniso-thickness. Its cytoderm is absence differently, but can be cultured in normal osmotic pressure media like HBSV and Hp media. The threadlike H. pylori was instability and can reversion to normal H. pylori while they were inoculated for fresh media. The study suggested that H. pylori variation can change from the typical 'S' form to coccoid and to long threadlike and then reversion to normal form. These changes may play a important role in H. pylori infection from environment or other body, or from themselves after treatment.