我国HCV感染株基因分型及其意义的研究

Genotyping of HCV isolates in China and It's Significance

应用型特异性探针杂交法及型特异性引物法两种ＨＣＶ基因分型方法对我国ＨＣＶ 感染株基因型别构成特点及其意义进行了初步研究。结果表明，１．我国 ＨＣＶ基因型表现为多种型别共存，但以 Ｋ１（１ｂ）型和Ｋ２（２）型为主，混合型比例亦较高；２．结合临床背景分析，Ｋ１型似与活动性肝病关系密切，慢性迁延性肝病及所谓合格献血员以Ｋ２型为主，混合型多与多次受血、透析有关；３．输血－受血配对研究结果提示，受血者ＨＣＶ基因型与所输注污染的血液或血制品ＨＣＶ基因型有良好的一致性，基因分型方法可以作为调查ＨＣＶ传播途径的重要手段。

Both type -specific prob assay and type- specific primer assay have been employed for the study of genotyping of hepatitis C virus isolates in China and it's significance. The result suggested that the character of the prevalence of HCV genotypes in China were: (1) each of the five kinds of HCV genetypes existed in China. The Orient Type (K1 or 1b)was the dominant type, and the prevalence of K2 and Mix types were higher than that in other countries. (2) analysed with their clinical background of the patients,K1 type was closely related to the activity of liver disease; K2 type was more frequently encounted in pa- tients with chronic persistent hepatitis and blood -donors;Mix type existed predominantly in patients receiving blood and dialysis frequently. (3) Matched study of transfusion re- ceiving blood showed that there was a good coincidence of HCV genotypes in the transfused blood or blood products and the blood of their receivers.