脂蛋白(a)和低密度脂蛋白对小鼠腹腔巨噬细胞内脂质的影响

The Effect Lipoprotein (a) and Low Density Lipoprotein on Lipid in Mouse Peritoneal Macrophages

将脂蛋白与巨噬细胞一起培养(每毫升培养液中含脂蛋白胆固醇200μg),24h后用薄层层析法测定巨噬细胞内胆固醇酯(CE)的含量.结果表明,天然脂蛋白(a)对巨噬细胞内CE的形成影响不明显(0.9μg/mg cell pro,p>0.05).LDL对巨噬细胞内CE的形成有促进作用(4.0μg/mg cellPro,P<0.05),LP(a)与LDL的混合物能明显地促进巨噬细胞内CE的形成(13.9/mg cell pro,P<0.01),其增加量是LDL的3.48倍.

Macrophages were incubated with Jipoproteins (lipoprotein cholesterol 200#g/ml medium) for 24h. The cholesterol ester (CE) in macrophages were detemined by thinlayer chromatograms. The result: native Lp (a) was not distinct on intracellar CE formation (0. 9μg/mg cell pro. , P&gt;0. 05); LDL increased CE formation in MPM (4. 0μg/mg cell pro. , P&lt;0. 05); The mixture of Lp (a) with LDL caused a remarkable increase in CE formation in MPM (13. 9μg/mg cell pro. , P&lt;0. 01). Which was 3. 48-fold as compare to LDL.