23个实验小鼠和大鼠近交系的保种研究

Studies on Maintenance of the Inbred Strains of Laboratory Mice and Rats

从1985年开始,陆续从国外实验动物著名机构引进常用近交系小鼠共23个品系(亚系和亚群)。在多年来保种繁殖过程中,根据修饰平行线系统选择留种动物,同时对动物进行全兄妹近亲交配繁殖,保持完整的谱系记录,控制饲养环境条件,成功地保持了绝大多数品系。同时对多数品系的繁殖性能和体重增长进行了观察,为这些品系的实验应用提供基本参数。

Sixteen inbred strains of mice and seven inbred strains of rats had been introduced from foreign countries since 1985, and were maintained in plastic film isolators in our institute. All of these strains, as foundation colonies, were kept according to a modified parallel line system with full brother-sister mating, accompanied with complete pedigree records including breeding record cards, pedigree charts and pedigree books. Up to now, except three mice and one rat strains which were extinct before 4 generations, all other strains survived to 11-22 generations. The environmental conditions for the animals were carefully controlled, including temperature, humidity, microbiological status and nutrition. The reproductive performance and body weight growth of these strains were observed, and data obtained here were different from previous reports to some extent. The results showed that many environmental factors could affect reproduction and development of the inbred strains. These effects must be taken into cosideration when the animals are used in long ter n experiments involved in animal reproduction and growth.