促吞噬肽和胸腺五肽对小鼠单核吞噬细胞功能的影响

The effect of tuftsin and thymopentin on phagocytic function in mice

研究了人工合成的内源性活性肽促吞噬肽(Tuf)和胸腺五肽(TP5)对吞噬细胞功能的刺激作用。Tuf刺激小鼠腹腔巨噬细胞(PMφ)和血清内溶菌酶(LSZ)及PMφ内酸性磷酸酶(ACP)活力升高,并呈剂量依赖关系;单次皮下注射给药时,血内LSZ水平升高,峰时在6天左右;不完全福氏佐剂(FIA)延缓并增强血内LSZ的升高作用。在环磷酰胺(Cy)免疫抑制小鼠模型,TP5使PMφ内Cy激活的酶活性进一步增高,使Cy抑制的脾LSZ活性恢复正常,提示TP5兼有激活吞噬细胞功能;又有恢复细胞数目的作用。

The stimulate effects of synthetical endogenous peptides, tuftsin (Tuf) and thymopentin(TP5), on phagocytic function were studied. After subcutaneous administration (sc) of Tuf, thelysozyme content in both Mφ and sera, and the acid phosphatase activity in Mφ, were increasedin a dose-dependent manner. The peak time of serum lysozme level following a sigle sc injectionof Tuf was about on the 6th day. The elevation of lysozyme in sera was delayed and enhancedstrongly by Freund incomplete adjuvant (FIA). In cylcophosphamide (Cy) treated mice,TP5further increased both lysozyme content amd acid phosphatase activity in Mφactivated byCy, and recovered the lysozyme level in spleen inhibited by cy. The results suggested thatTP5could not only activate the funceion of Mφ, but recover the number of Mφdecreased by im-mumoimhibitant.