人体T细胞CD_8^+VV^+亚群在活动性SLE患者中显示反抑制活性

Contrasuppressive activity of the T cell Subsets with vicia lectin receptor in systemic lupus erythematosus

反抑制T 细胞(TCS)借助T 辅助细胞对抗T 抑制细胞的抑制效应,参与免疫调节。小鼠和人体的TCS 都表达长绒毛野豌豆凝集素受体(VV—R)。人体TCS 则主要是CD_8^+VV^+T 亚群,和临床多种疾病免疫功能异常密切相关。本文用抗VV 抗体单色和双色免疫荧光法和流式法细胞术(FCM)测知正常人VV^+T 亚群的比例是16.6±2.9%。用FCM 双色免疫荧光法检测到活动期SLE 患者CD_8VV^+亚群百分比高达23.63%和对照组(8.50%)及稳定期(14.49%)相比差异显著。然而CD_4^+VV^+亚群在三组SLE 中基本不变。进一步深入分析是做有关CD_8VV^+T 细胞亚群的功能测定,证实活动性SLE 患者VV^+T 细胞具有比正常个体高得多的反抑制活性,相对反应值从100%提高到485%和625%。提示CD_(?)VV^+细胞和SLE 免疫调节功能紊乱密切相关。

Contrasuppressor T cells (TCS) take part in immunoreglation by rendering T help cells re-sistant to the effects of suppressor cells.It was found that both human and mouse TCS expressreceptor for vicia villola letcin (VVR).Human effective TCS are mainly CD8VV^+ T subsetswhich are closely associated with abnormalities of immune activity of many diseases.By usingroutine and double immunofluorescent labelling techniques combined with flow cytometry,dif-ferentVV^+ subsets were analysed.A significant increase was observed for CD8VV^+ cells in ac-tive SLE (23.6%)as compared with the control group (8.5%) and the SLE patients in remis-sion (14.49%).The percentage of CD4VV^+ subset,however,was unchanged.Moreover,thetypical contrasuppressive activities exerted by VV^+ cells,which were also increased significantlyin active SLE patients,were detected in SLE in a dose—dependent manner.In an experimentwith PPD—induced lymphoproliferation as indicating system,for example,the relative respons-es were observed to be increased doses in from 100% to 485% and 625% after the VV^+ cellswere added back in increased doses,suggesting that the contrasuppressive VV^+ or CD8VV^+cells were added back in increased doses,suggesting that the contrasuppressive VV^+ or CD_8VV^+cells Were closely related to the immune disorders of SLE.