补体C_3的自然转换特征及rIFN-γ和SPG对C_3的调节

Characteristics of Natural transformation of C_3 components and regulation of rIFN-γ and Sizofiran on pro-C_3

用免疫印迹方法观察了补体C_3从pro-C_3自然转换到C_(3c)的过程以及重组干扰素-γ(rIFN-γ)和抗肿瘤多糖Sizofiran(SPG)对C_3的调节。在4℃时,血清中Pro-C_3完全降解为C_(3C)约需18天,C_3与C_(3b)的水平在pro-C_3降解过程的第8天为最高。rIFN-γ和SPG 均能诱导pro-C_3的合成,两者合并使用效果更显著。上述结果提示C_3在体内是以pro-C_3的形式存在的,大部分的物质对补体成分C_3的影响亦是改变pro-C_3的合成。由于C_3随着血清贮存的时间不断变化,因而对补体C_3分析获得的结果都拟被视作是“瞬时结果”。

1.Shanghai Institute of Physiology,Chinese Academy of Sciences.Shanghai 200031.2.Central Research Laboratories,Kaken pharmaceutical Co.,Ltd.Tokyo 107.3.Department of Gynecology,Cancer Institute Hospital.Tokyo 117.The natural transformation of C_3 components and regulation of rIFN-γ and/or SPG on pro-C_3 were observed by immuno-blotting analysis.Under the condition of sera stored at 4℃,the pe-riod of transformation from pro-C_3 to C_(3(?)) was about 18 days.The highest level of fragment C_3and C_(3b) appeared on day 8 during the degradation of pro-C_3.Level of pro-C_3 could be enhancedby administration of rIFN-γ and/or SPG,and the most potent means was to take the method ofrIFN-γ and SPG in combination.These results were suggested that so called the third compo-nents of complement in vivo was in pro-C_3 form in reality,and most reagent was to affect on thesynthesis of pro-C_3 instead of other C_3 components.In addition,all obtained results about the lev-els of C_3 components should be considered as an“instantaneous result”due to the C_3 componentsvaried with the store time-course of sera.