摘要
本文报告一种新型试管固相RIA和IRMA技术。先后建立了固相二抗测定β2-mG,固相一抗法测定AFP和双位点夹心法测定CEA。实现了分析技术一步法。本法操作十分简便,不加分离剂和离心程序,不受温度影响,便于组装试剂盒及临床大量样品的检测。这一技术的改进成功为我国放免试剂盒的更新换代提供了条件。
A new type of testtube solid-phase radioimmunoassay and immunoradiometric assay was
proposed and examplified by β 2-mG、AFP and CEA determinations.A solid-phase antibody
technique was introduced in AFPassay,Solidphase second antibody was used in β2-mGdetermi-
nation and two-site sandwich format was employed in CEA assay.All these methods utilized
one-step protocol with no centrifugation needed.The proposed methods are accurate,convinent
to operate and suitable for automatic high throughput sample mmeasurement.This achievement
might pave the way for a new generation RIA。
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1993年第4期223-225,共3页
Chinese Journal of Immunology