SPA菌体协同ELISA及其在检测血清抗HCV·IgM中的初步应用

Establishment and Application of SPA Co-operated ELISA for the detection of Anti-HCV-IgM

采用HCV_1合成抗原多肽,SPA 菌体及酶标记抗人IgM 等建立血清抗HCV.IgM 检测SPA菌体协同ELISA(SPA-ELISA)。以多重替代试验、阻断试验及其2-巯基乙醇破坏试验证实其方法的特异性.将其用于一组临床标本的检测,并对该法检测血清抗HCV-IgM 的优点进行了探讨。

A Staphylococci aureus protein A co-operated ELISA (SPA-ELISA)for the detection ofanti-HCV-IgM has been established using HCV antigenic polypeptide,SPA bearing germ andhorseradish peroxidase labeled anti-human IgM.The specificity of SPA-ELISA has been con-firmed by some substitution tests,blocking test and destroying test with 2-mecaptoethanol.Theexperimental results showed that the positive rate of anti-HCV-IgM was higher than that of an-ti-HCV-IgG in a group of patients with acute hepatitis and there were predominant differencesof anti-HCV-IgM detected rates between patients with acute hepaetitis (61.54%)and patientswith chronic hepatitis (32.26%),(P<0.01).On the other hand,the positive rates of anti-HCV-IgM and anti-HCV-IgG were 53.66% and 63.41% in blood transfusion associated hepati-tis,38.10% and 42.86% in sporadic hepatitis,6.11% and 16.33% in population with activesocial activities,4.0% and 10% in a group of blood donors.Furthermore,considering the quali-ty of HCV polypeptide,handling witn ease,and excluding the interference of anti-HCV-IgG insera,the new SPA-ELISA is believed to has a practical value in the clinical and epidenmiologicalstudies of hepatitis C.