斑点免疫金银染色检测RNA抗体及其临床意义

Detection of anti-RNA antibody by dot-immunogold silver staining and its clinical significance

采用斑点免疫金银染色法检测了280份临床血清标本的抗RNA 抗体.其中144份血清同时以ELISA 法作平行对照。两法检测结果差异无显著性(P>0.05),符合率91%,两法定量结果间存在高度的直线正相关性。该抗体在自身免疫病中的检出率为46.6%,其中在SLE 达62%,但在非自身免疫病仅为5.1%,可作为自身免疫病筛查指标,对ANA 阴性者尤有意义。

Anti-RNA antibodies(ARNA)were determined by dot-immunogold silver staining(Dot-IGSS)in 280 clinical serum samples.144 of these sera were tested simultaneously by ELISA ascontrast.The coincident rate of the detection results obtained from Dot-IGSS and from ELISAwas 91% and the difference between these results was insignificant in statistics.Moreover,therewas a positive linear correlation between the results Of quantitative analysis by the two methods.The positive rate of ARNA was 46.6% in group of autoimmunodiseases(AID),while 5.1% inthe group of non-AID and normal persons and ARNA could be regarded as another lead forscreening AID,especially for ANA-negative patients.