一种新的去大鼠淋巴结输入淋巴管的方法——ZT胶法

ZT TISSUE ADHESIVE PAINTING METHOD:A NOVEL PROCEDURE OF DEAFFERENTIZATION FOR RAT LYMPH NODES

采用一种新的去输入淋巴管并防止其再吻合的方法——ZT 胶法,分别于术后1d、10d、20d、28d、和55d 对大鼠颈淋巴结进行观察。术后淋巴结实质内的淋巴细胞数、生发中心数、ACP 强阳性的巨噬细胞数均逐渐下降,残留的巨噬细胞出现衰老症象,高内皮微静脉的内皮细胞逐渐变扁平,嗜派洛宁性逐渐减弱。这些变化与以前报道的结果基本一致,表明该新方法可靠。在实验期限内,阻断输入淋巴管的成功率几乎可达100%。本研究为今后开展有关去输入淋巴管的研究提供了一种极为简便而可靠的方法。

The morphological changes of rat cervical lymph nodes were observed at 1,10,20, 28 and 55 days after operation which had been done with a novel procedure of deaffe- rentization by painting a full layer of ZT tissue adhesive on the surface of the lymph nodes deprived of afferent lymphatics to prevent reafferentization.We found that after operation,the number of lymphocytes,germinal centers and ACP-strong-positive mac- rophages of the lymph nodes was reduced gradually.The remaining macrophages had the characteristic of senility.The endothelial celts ol high endothelial venules became flattened,and their affinity for pyronin decreased.These changes are consistent with the reported results,indicating that our new technique is reliable.Within the experi- mental deadline,the success rate of deafferentization is almost 100%.Our experiments provide an extremely simple and reliable method for future research concerning deaffe- rentization.