乙二胺所致哮喘的临床免疫学研究

The Clinical Immunologie study of Asthma Induced by Ethylenediamine

对5例EDA哮喘患者做了非特异性支气管激发试验,结果气道反应性轻度增高2例,中度增高3例。8例EDA哮喘患者做了特异性支气管激发试验,结果全部阳性,其中7例为迟发相反应,1例为双相反应。而普通哮喘组和正常对照组激发试验全部阴性。激发试验前后测定了总补体、组胺,作了嗜酸细胞计数。激发后EDA哮喘组嗜酸细胞明显增加,并有统计学意义(P<0.05),与普通哮喘组和正常对照组激发后嗜酸细胞计数比有显著差异(P<0.01)。EDA哮喘组激发后组胺释放增加(P<0.05)。EDA哮喘组总补体均未见有意义的改变。EDA-BSA皮内试验结果表明,EDA哮喘4例为阳性,普通哮喘组1例为阳性,而对照组无1例阳性。EDA-BSA作抗原,以酶联免疲分析证明了EDA哮喘组有3例有特异性IgG抗体,而其他两组未发现特异性IgG抗体,EDA哮喘组未检出特异性IgE抗体。

The first group consisted of 8 asthmapatients induced by EDA, the second were5 common asthma patients, and the thirdalso had 5 people used as normal control. Non-specific provocation test was carried outin 5 subjects of EDA asthma. Two of themshowed slight reaction, there had moderatesreaction. Specific provocation test (SPT) waperformed in 8 cases of EDA asthma with0.1% EDA. All showed positive responses.7 of them were late type response, anotherwas a dual response, while common asthmaand normal control groups showed negativeresponses in BPT. Total complement, histam-ine and eosinophil counts were investigatedprior to and after the test. Eosinophil countswere increased in EDA asthma group, (P<0.05), and had significant difference withthe other two groups, (P<0.01). Histaminein EDA group was also increased after thetest. The difference was significant (P<0.05). There were no significant changesin the other two groups (P>0.05). Totalcomplement in EDA group had no significantchange. 4 patients in EDA group showedpositive results in the skin-prick test, andone of the common asthma group showed po-sitive result as well, who had a history oftaking aminophylline. The positive results ofthe skin-pride test indicated that there wereantibodies of EDA-BSA. ELISA analysis pro-ved that 3 of the EDA asthma group possessedspecific IgG antibodies, while in the other 2groups, specific IgG was not found. SpecificIgE antibodieswere not found in EDA asthmagroup. Being the first report in the literature.This study suggests that the pathogenesis of some EDA. late type asthma might be animmunologic response mediated by IgG.