皮肤鳞癌组织中凝集素受体的研究

Study on Lectin Receptors in Squamous Cell Carcinomas.

应用ABC免疫组化方法对皮肤鳞状细胞癌(SCC)和角化棘皮瘤(KA)的6种凝集素受体表达进行了研究。结果显示:花生凝集素(PNA)受体和蓖麻凝集素(RCA-1)受体在SCC和KA的表达明显不同,用神经氨酸苷酶消化前SCC中绝大部分无PNA受体表达,而消化后则有PNA受体表达。提示SCC中PNA受体未见表达是因为唾液酸残基封闭了PNA受体,而并非缺少PNA受体。PNA和RCA-1受体在SCC和KA中表达上的差异性可用于鉴别诊断。

Receptors of 6 kinds of lectin in squamous cell car cinoma ( SCC ) and keratoacanthoma (KA) were studied by ABC method. The results showed that the expressions of PNA and RCA-1 receptors were significantly different between SCC and KA. There was almost no PNA receptor expression in SCC, but after digestion with neuraminidase, PNA became positive in SCC. It suggested that no PNA receptors expression do not result from lack of PNA receptors, but are due to the masking of receptor site by terminal residues of sialic acid. The differences of PNA and RCA-1 expression are useful in differential diagnosis between SCC and KA.