川东獐牙菜药材的HPLC指纹图谱初步研究

Preliminary studies on HPLC fingerprint of Swertia davidi Franch

目的 研究川东獐牙菜药材的HPLC指纹图谱。方法 色谱柱为大连依利特公司HYPERSIL BDS C_(18)(4.6mm×200mm,5μm)。流动相为乙腈-磷酸二氢钠(0.03 mol·L^(-1),pH=3.0),梯度洗脱(ACN％:0 min10％,15 min 20％,30 min 35％,50 min 55％),柱温为25℃,流速为1.0 mL·min^(-1),检测波长为250 nm。结果 初步建立了川东獐牙菜药材的HPLC指纹图谱,标定了14个共有指纹峰、并利用对照品对7个共有指纹峰进行了标记、结论 利用川东獐牙菜HPLC指纹图谱可以较为全面地反映川东獐牙菜药材的内在质量,有助于该药材的标准化种植。

OBJECTIVE To determine the HPLC fingerprint of the Swertia davidi Franch . METHODS Chroma-tographic column: Hypersil BDS C18 (4.6 mm×200 mm. 5 μm). Mobile phase: ACN-NaH2PO4 (0.03mol· L-1, pH = 3.0) . Gradient elution: ACN%: 0min 10%. 15 min 20%, 30 min 35%, and 50 min 55%. Column temperature: 25℃. Flow rate: 1 mL·min-1. Wavelength: 250 nm. Inject volume: 10 μL. RESULTS The fingerprint of Swertia davidi Franch was established and 14 common peaks in the fingerprint were indicated. Through reference substances. 7 common peaks were identified. CONCLUSIONS The HPLC fingerprint can be used to control the quality of Swertia davidi Franch.