失血性休克大鼠血浆β-内啡肽和一氧化氮含量变化及其联系

Changes and relationships of the concentrations of β-endorphin and nitric oxide in hemorrhagic shock in rats

目的 了解在失血性休克时β -内啡肽 ( β -EP)和一氧化氮 (NO)之间血浆含量的变化是否存在某种联系并探讨其机制。方法 12只SD大鼠麻醉后经股动脉放血复制失血性休克模型 ,分别在 5个不同时点采集大鼠血浆 ,通过生物化学方法检测其中 β -EP、NO和NOS的浓度。 结果 失血性休克后血浆 β -EP、NO和NOS的浓度都显著升高 ,并且NO与 β -EP的浓度变化存在一定的正相关 ,与NOS的浓度变化在失血性休克后期存在显著的正相关。 结论 ①β -EP和NO在失血性休克中可能都扮演重要角色 ;②在失血性休克后期血中NO浓度的升高主要是由iNOS所产生的 ,提示iNOS对失血性休克的发展和预后起着关键性作用 ;③同时本研究的结果也提示在失血性休克时 β

Objective The purpose of this study is to find if there are some relationships of plasmic concentration's alteration of β-endorphin(β-EP)and nitric oxide(NO) in hemorrhagic shock(HS) and their mechanisms Methods 12 Sprague-Dawley rats were anesthetized and subjected to induce HS by femoral arterial catheter.The plasma was collected at five different stages and the concentrations of β-EP,NO and NOS were respectively tested by different biochemical assays.Results The concentrations of β-EP,NO and NOS were all significantly elevated after HS. And the plasmic concentration's alteration of NO was similar to one of β-EP,and was significant positive correlative to one of NOS in late HS.Conclusions ①β-EP and NO may all play important roles in HS;② The enhanced formation of NO in late HS is mainly produced by iNOS.This suggests that iNOS may be a key to the development and the prognosis of HS;③The results also suggest that β-EP and NO may be mediated by one another,and may be in synergy to exert effects in HS. [