纳洛酮在大鼠肠缺血再灌流致肺损伤中保护作用的初步研究

Experiment study on protective effects of naloxone on lung injury from intestinal ischemia-reperfusion

目的 探讨纳洛酮在大鼠肠缺血再灌流致肺损伤中的作用。方法 成年雄性SD大鼠 ,随机分为以下四组 :假手术组、肠缺血再灌流组及分别用生理盐水和纳洛酮干预后的肠缺血再灌流组。分别测各组大鼠血浆 β -内啡肽及肺组织丙二醛含量、髓过氧化酶活性和肺毛细血管通透性。结果 肠缺血再灌流组血浆 β -内啡肽及肺组织丙二醛含量、髓过氧化酶活性和肺毛细血管通透性高于假手术组 ,而用纳洛酮干预后各指标测定值较生理盐水对照组明显降低。

Objective To investigate the protective effects of naloxone on lung injury from intestinal ischemia-reperfusion.Methods 32 adult male SD rats were randomly divided into four group (n=8), such as sham-operation, intestinal ischemia-reperfusion( IIR), IIR treated with normal saline (NS control), and IIR treated with naloxone (NX treatent). The plasma levels of β-endophin (β-EP) and levels of malondialdehyde (MDA), myeloperoxidase( MPO) of the lung, as well as pulmonary microvascular permeability were checked in each group. Results The level of β-EP, MDA, MPO and pulmonary microvascular permeability were significantly higher in the group of IIR than sham-operation group. After NX treatment, these parameters of the NX group were significantly lower than NS control group. Conclusion NX showed a protective effects on lung injury from IIR. [