高效液相色谱法测定苹果果皮的类胡萝卜素

The Determination of Carotenoids in Apple Peel by HPLC

用高效液相色谱法测定苹果果皮多种类胡萝卜素。以丙酮与石油醚提取色素 ,用NovapakC18(4 .6mm×15 0mm)色谱柱 ,乙腈∶甲醇∶二氯甲烷 (70∶2 0∶10 )流动相 ,在 45 0nm下检测类胡萝卜素。结果表明 ,玉米黄质、叶黄素、番茄红素与 β 胡萝卜素的回收率分别是94.2 7%～ 99.82 %、84.5 6 %～ 89.41%、87.5 3～ 93.5 4%与 88.0 8%～93 .2 4%。其中富士果仅检测到玉米黄质 (5 .9× 10 -3 μg/mm2 )与 β 胡萝卜素 (5 .4× 10 -3 μg/mm2 ) ;蛇果 (红星 )只有玉米黄质 (9.1× 10 -3 μg/mm2 )。

A reversed phase high performance liquid chromatographic method was used to determine carotenoids in apple peel.Carotenoids were extracted from apple peel with acetone and petroleum ether.Using Novapak C 18 column( 4.6 mm ×150 mm),acetonitrile∶methanol∶methylene chloride (70∶20∶10) as mobile phase,carotenoids were eluted and monitored at 450 nm.It was shown that recoveries of zeaxanthin,lutein,lycopene and β carotene were 94.27 %～ 99.82 %, 84.56 %～ 89.41 %, 87.53% ～ 93.54 %,and 88.08% ～ 93.24 %.‘Fuji’ apple contained zeaxanthin ( 5.9 ×10 -3 μg/mm 2) and β carotene ( 5.4 × 10 -3 μg/mm 2 ) and Red Delicious apple only zeaxanthin ( 9.1 ×10 -3 μg/mm 2).