反相高效液相色谱法测定溃结灌肠液中没食子酸和绿原酸的含量

Quantitative Determination of Gallic Acid and Chlorogenic Acid in Kuijie Enema by RP-HPLC

用反相高效液相色谱法,以甲醇:冰醋酸:二甲基甲酰胺:水(5:1:25:169),甲醇:冰醋酸:水(15:2:83)为流动相测定溃结灌肠液中主要有效成分没食子酸和绿原酸的含量,结果提示:溃结灌肠液中没食子酸含量为0.14～0.21％,绿原酸含量1.30～1.41％,没食子酸回收率为99.82％,变异系数为1.65％,绿原酸含量为99.51％,变异系数为1.05％。

The content of gallic acid and chlorogenic acid in Kuijie Enema was determined by RP- HPLC. The mobile phase was methanol : glacial acetic acid : dimethyl formamide : water(5 : 1 : 25 : 169)and methanol : glacial acetic acid : water(15 : 2 : 83) respectively, The results showed that the content of gallic acid and chlorogenic acid is 0. 14%~0. 21%,1. 30~1. 41%, the recovery is 99. 82%、99. 51 % ,and the cofficient of variation is 1. 65%、1. 05% respectively.