多价鼠肝炎病毒抗体ELISA试剂盒的研制及应用

Preparation and Application of An ELISA Kit for Detection of MHV Antibody Using Polyvalent Antigen

从6株鼠肝炎(MHV)病毒中筛选出A_(59),MHV_1及沪-79(Sh-79)3个毒株制成多价抗原的ELISA试剂盒。检测SPF和清洁级小鼠血清104份,又为0.037,SD为0.024,阳性临界值为0.207。经电镜检查,阻断抑制试验和IFA验证,证明本试剂盒特异性好。应用本试剂盒检测实验鼠群MHV抗体的敏感性单价抗原强。开放鼠MHV抗体阳性率为72％(161/223),Ⅱ级和Ⅲ级鼠为1.7％(2/117)。

An ELISA kit using polyvalent antign (A59, MHV1 and Sh-79) screened out from 6 strains of MHV was prepared. 104 serum samples of SPF and clean level mice were detected for MHV antibody by the kit and the result showed that x, SE and positive cut-off value were 0. 037, 0. 024 and 0. 207, respectively. The specifcity of the ELISA kit was detected by electron mcroscope, blocking inhibition test and IFA test and proved to be good. The sensitvity of detection of MHV antibody by the ELISA kit was higher than that of by monovalent antigen. The positive rate of antibodies of ordinary mice was 72% (161/223), and that of mice of class I and Ⅲ was 1.7%(2/ 117).