摘要
目的：观察白细胞介素-2（Interleukin-2，IL-2）对淋巴细胞体内迁移的影响。方法：选用近交系小鼠为实验动物，背部剃毛，皮内注射细胞因子，同时尾静脉输注3H-UR标记的淋巴细胞。20h后，切取注射区皮块，制样后测定样品计数率。经计算后将计数率转换成细胞浓度因子（Cell concentration factor ，CCF），用CCF值反应淋巴细胞迁移的水平。结果：IL-2注射区淋巴细胞迁移量与对照组无明显差异（P>0.05），而且IL2与肿瘤坏死因子α联合应用时，亦没有协同作用。结论：提示在复杂的体内环境中，IL-2对小鼠脾淋巴细胞体内迁移无明显诱导作用。
examine the effects of interleukin-2 on lymphocyte mig ration in vivo Methods: BALB/C mice were choosed as experime ntal animals The s kin on the backs of all mice was shaved, cytokines or the mixture of cytokine was injected intradermally At the same time , spleen lymphocytes labelled with 3 H-UR were i.V injected 20 hours later, the injected areas were punched out Through emulsion counting, the counts per minute (CPM) of 3H were examined After calculating, the cell concentration factor (CCF) was get Results: All of CCF of different doses of IL-2 have no significant difference c ompared to the control (P>0.05) The CCF of combination of IL-2 and TNF α was 71% of the sum of IL-2 and TNFα alone
