摘要
利用聚合酶链反应(PCR),对从猪细小病毒(PPV)三株强毒及 两株弱毒的细胞培养产物中提取的核酸进行扩增。通过琼脂糖电泳,均得到了约158bp(碱基对)的特异性核酸片段。与该病毒本身的二个含20bp的寡核苷酸引物之间的核酸长度相等。而对与PPV具有基因同源性的犬细小病毒(CPV)及与PPV临床症状相似的非相关病毒如日本乙型脑炎病毒(JEV)、伪狂犬病毒(PRV)作同样处理,未得到核酸带。并且通过筛选裂解蛋白的方法,建立了一种快速提取DNA的方法,初步制定了快速检测PPV的程序。
The nucleic acids extracted from the cell cultures of 3 virulent and 2 aviruleat strains of. porcine parvoviruses (PPV) were amplified with PCR. By agarose electrophoresis, the specific nucleic acid fragments of 158 base pairs (bp) all were obtained from them, which length was as same as the nucleic acid's between the two oligonucleotide primers containing 20 bp. But the nucleic acid bands were not gained from canine parvovirus (CPV), Japanese B encephalitis virus (IEV) and pseudorabies virus (PRV) in spite of giving the same treatment. The auther established a method for rapidly extracting virus DNA and provided a program for rapidly measuring PPV. ?
出处
《中国兽医科技》
CSCD
1993年第3期3-4,共2页
Chinese Journal of Veterinary Science and Technology
基金
北京市自然科学基金
