新生儿脐带血中粒-单系造血祖细胞数量及性质的研究

Study of Quantity and Quality of Colony Forming Unit-Granulogyte and Macrophase(CFU-GM)in Neonate Cord Blood

采用单层琼脂法对30例足月新生儿脐带血造血祖细胞(CFU-GM)进行了培养,并比较了脐血、成人骨髓和外周血 CFU-GM 的数量和性质。结果表明:①新生儿脐血中 CFU-GM 分裂快,集落形成时间早,集落体积大,子代细胞含量多,表现为活跃的增殖状态。②足月新生几脐血 CFU-GM 和Cluster 的产率介于成人骨髓和外周血之间,但个体差异较大;13.3%的脐血样本,CFU-GM 产率高于同时培养的骨髓。此外,脐血、成人骨髓、外周血中 CFU-GM 集落类型不同,提示脐血 CFU-GM 可能是一种不同的亚型。

The CFU-GM in 30 full-term newborn cord blood were cultured on the mon-olayer agar and compared with those,in quatity and quality,of cord blood,adultbone marrow and peripheral blood,indicating:1.The CFU-GM in newborn cordblood were in a state of the active proliferization with characterization of rapiddivision,early colony appearance,largy colony volume and more filial cell con-tainment;2.The yield of CFU-GM and cluster in full-term newborn cord blo-od ranged between those in the adult bone marrow and peripheral blood but variedgreatly from one individual to another,the yield of CFU-GM in 13 3% cordblood samples was higher than that in the bone marrow cultured at the same time.In addition,the colony type of CFU-GM in cord blood,adult bone marrow andperipheral blood was different,suggesting that the CFU-GM in cord blood mightbe a different subtype.