放烧复合伤后输贮存血对异体植皮大鼠免疫反应的影响

ENGLISH ABSTRACTS OF ORIGINAL ARTICLES——Effect of Transfusion with Stored Blood after Combined Radiation-Burn Injury on Immune Reaction of Allo-Skin Grafted Rats

本文用烧伤(15%Ⅲ度)和放烧复合伤(5Gy 全身照射+15%Ⅲ度烧伤)大鼠异体植皮的模型,探讨了伤后输贮存血对移植免疫反应的影响。烧伤植皮后,早期细胞免疫功能明显增强,皮片于7天内全邗死亡;加输贮存血后,细胞免疫功能增强程度降低,但对皮片存活影响不显著。而放烧复合伤植皮加输贮存血时,胸腺细胞增殖能力和脾细胞抗体形成能力受抑重、时间长,胸腺和脾脏细胞数以及脾 B、T、T_H、T_S 细胞数下降快、恢复慢,皮片15天活存率(100.0%)显著高于单给植皮组(28.6%,P<0.01)。提示放烧复合伤异体植皮时输贮存血具有抑制细胞免疫应答反应增强的作用,从而使异体皮活存时间延长,有利于整体伤情的治愈。

The effect of post-injury transfusion with stored blood on the immune reactionof allo-skin grafted rats was treated,using models of the allo-skin grafted ratswith simple burn injury (15% TBSA) and with combined radiation-burn injury(exposed to 5 Gy systemic irradiation,15% TBSA) respectively.After burninjury and allo-skin graftment,the early cell immune function of the rats signifi-cantly enhanced,the survival time of all the allo-skin grafts was within 7 days.After the rats transfused with stored blood,the degree of their cell immunefunction enhancement was decreased,but there was no significant effect on thesurvival time of allo-skin grafts.However,in the case of allo-skin graftmentsubsequent to combined radiation-burn injury,and then transfusion with storedblood,the multiplying ability of thymocytes and the antibody-producing abilityof splenocytes were depressed significantly and for a long time;the number ofthymocytes,splenocytes,spleen B-cells,T-cells,T_H-cells and Ts-eells were decr-eased rapidly and recovered slowly.And the surviral rate of allo-skin grafts for15 days was 100.0% which was remarkably higher than that of the group subject-ed to simple allo-skin graftment (28.6%,P<0.01),indicating that transfusionwith stored blood after combined radiation-burn injury and allo-skin graftmentplayed a role in depressing the enhanced immune reaction of the rats,which cou-ld lead to the prolonging of survival time of allo-skin grafts and was beneficialto the cure of whole injury condition.