清洁级实验动物细菌学检测用主要培养基的筛选

Selection of Culture Media in Bacteria Detection of Cleaning Laboratory Animals

目的 为解决实验动物细菌检测用培养基标准化的问题 ,选择目前国内几个比较大的培养基生产厂家和一个国外知名培养基生产厂家的DHL、SS、营养琼脂 3种培养基 ,利用已知标准菌株进行对比、优选 ,从而形成标准产品 ,最终确定适合于实验动物细菌检测用的培养基。方法 用标准沙门菌、嗜肺巴斯德杆菌、鼠棒状杆菌对培养基进行定性 (生长实验 )和定量 (平板菌落计数实验 )筛选。结果 6个厂家的 3种培养基除SS琼脂有差异外 ,其余均无显著差异 ,Merck公司的血琼脂上嗜肺巴斯德杆菌未能生长。结论 在实际检测应用中Merck公司的血琼脂不能选用。而Merck公司和中国药品生物制品检定所生产的SS琼脂对细菌有较强的抑制能力 ,在实际检测中有可能造成漏检 ,也不宜选用 ;

Objective To standardize the det ection culture media for bacteria in cleaning laboratory animals, three kinds o f media (DHL agar, SS agar, nutrient agar) from 6 media manufactures were selecte d. Methods 3 strains of bacteria ( Salmonella, Pasteurella Pne umot ropica, Bordetella Bronchiseptica) were used to compare and contrast quantitat ively and qualitatively. ResultsThere was no difference among the three kinds of m edia from 6 media manufactures except SS agar. SS agar from National Institute f or the Control of Pharmaceutical and Biological Products and MERCK Inc. had a s trong suppressive effect to Salmonella. Pasteurella Pneumotropica could not grow at blood agar from MERCK Inc. ConclusionBlood agar from Merck Inc. are not suitable . SS agar from National Institute for the Control of Pharmaceutical and Biological P roducts and Merck Inc are not suitable too. The remainders are eligible.