用小型猪研究心肌缺血再灌注损伤的机制

STUDY ON THE MECHANISMS OF MYOCARDIAL ISCHEMIA AND REPERFUSION INJURY IN MINIATURE PIG MODEL

应用小型猪在体心脏缺血再灌注(IR)模型,探讨心肌IR损伤机制。发现心肌IR后有下列变化:①心肌细胞内钙、钠增多,钾减少。②心肌细胞膜流动性降低及冠状窦静脉血化学发光增强,间接显示自由基产生增多。③白细胞激活后产生氧自由基的反应增强,提示白细胞可能参与IR损伤机制。

An in vivo porcine heart ischemia and reperfusion model was used to study the mechanisms of myocardial ischemia and reperfusion injury. The results showed1 In ischemic and reperfused myocardium, Na and Ca significantly increased and K declined.2 Chemiluminescence of coronary sinus blood during reperfusion was increased significantly. The fluidity of sarcolemma of myocardial celt in the ischemic zones was decreased. These results indirectly demonstrated increased free radical production during reperfusion and myocardial damage caused by free radicals.3 Using ESR technique, it was found that during ischemia and reperfusion, oxygen free radicals produced by WBC respiratory explosion increased. This demonstrated that WBC participates in myocardial ischemia and reperfusion injury and WBC is one of the sources for oxygen free radicals.