薄层层析紫外分光光度法测定蜂王浆制剂中10-HDA的含量及其结果分析

Determination of the Levels of 10-HDA in Royal Jelly Preparations Using TLC-UV Spectrophotometry

本文报道用薄层层析紫外分光光度法测定蜂王浆制剂中10-羟基-△~2-癸烯酸(10-HDA)的含量。方法快速,准确度和重现性尚好,对设备条件要求不高,能起到控制质量的作用,亦可作为其它含蜂王浆制品中10-HDA的定性、定量分析方法参考。本文对39批市售蜂王浆制剂的测定结果提示,10-HDA的检出阳性率很低。故指出制定这类制剂的10-HDA的定性鉴别和含量测定指标是非常必要的。

This paper reports the determination of 10-hydroxy-trans-△~2-decenoic acid(10-HDA) levels in royal jelly preparations using TLC-UV spectrophotometry. This method was accurate, fast and simple with a CV of 12. 7%, offering a tool for drug control. It can be used in qualitative of quantitative analysis of 10-HDA in any royal jelly preparation. Thirty-nine royal jelly preparatons were determined and the results showed that 10-HDA was found only in 51. 3% of them. It seems to be necessary to work out a standard for qualitative identification and quantitative determination of 10-HDA in royal jelly prepartions.