乙肝病毒核心抗原基因在大肠杆菌中的高表达

High Expression of Hepatitis B Core Gene in E. coli

乙肝核心抗原在大肠杆菌表达由Michael Nassal完成,国内马贤凯等人成功地使乙肝核心抗原在Lac启动子控制下高表达。本文使乙肝核心抗原基因在P_LP_R启动子控制下,用温敏法诱导产生HBcAg,该菌株能稳定地高表达。可以代替肝提取的HBcAg。

HBc gene was isolated from whole long HBV DNA and digested with nuclease BAL 31, and then dNTP, DNA polymerase I(L. F), phospharylated EcoRI linker were added. The HBc DNA was digested by EcoRI and was then inserted into the EcoRI site of pJLAS02 plasmid. Recombination DNA was then transformed into E. coli JM 103. High expression recombinant was selected and named E. coli pJLA502 detected by argrose gel electrophersis. The HBcAg produced by recombinant and extracted by dead body liver was determined with ELISA and observed with electro micrograph. The HBcAg was very specific and may be used in clinical detecting kit.