黄芩前体脂质体再复水后的新脂质体粒径研究

Studies on the Size of Rehydrated New Liposome from Scutellaria Proliposome

用超声波法或高压乳化法等制备黄芩脂质体分散液,该脂质体粒径50～90 nm,包封率和回收率均达100%。用流动床将它向各种糖类芯料(山梨醇、葡萄糖、甘露糖、蔗粒、乳糖等)作切线喷雾制成粉末——黄芩前体脂质体。在喷雾过程和再复水过程中,原脂质体的有效碰撞几率可因非挥发性高沸点介质而减少,再复水溶媒中的磷酸根离子会使新脂质体粒径增大。减少或控制这些因素可制得性质稳定、不易氧化的黄芩前脂质体,其再复水形成的新脂质体粒径在100 nm左右,符合注射要求。

The scutellaria liposome dispersion solution was made by supersonic waves and high pressure homogenization methods. Its vesicle size is 50—90nm, and the entrapment and withdrawal proportion are all nearly 100%. The liposome was made into the powder—scutellaria proliposome with the fluid bed by tangential spray way to jet it upon the various core matter of carbojydrate (sorbitol, glucose, mannitol, sucrose, lactose). In the processes of spraying and rehydrating, the effective bump probability of original liposome was decreased due to the existence of the unvolatile high boiled point medium. In the rehydrated solution, the size of new liposome was increased by the ion of phosphate. The stable scutellaria proliposome can be prepared by controlling and reducing these affected factors. The rehydrated new liposome size is about 100nm, and it is suitable for injection.