胶束电动毛细管色谱法对三磷酸腺苷及其分解产物的分离分析

Analysis of Adenine、ATP、ADP、AMP and cAMP by Micellar Electrokinetic Capillary Chromatography

对ATP(adenosine triphosphate)及其分解产物ADP(adenosine diphosphate)、AMP(adenosine monophcsphate)等采用胶束电动毛细管色谱法进行分离分析。电动色谱系统为:毛细管柱57 cm×45 μm(I.D),石英毛细管,UV紫外检测器(波长260 nm),磷酸盐缓冲溶液(Na_2HPO_4)5 mmol/L,十二烷基硫酸钠(SDS)50 mmol/L(pH=9.06)。获得了良好的分离,为ATP的定量建立了良好的分析系统。

Adenine、ATP、ADP、AMP and cAMP were separated by Micellar Electrokinetic Capillary Chromatography (MECC) with UV detection at 260nm on a 57cm×45μm ID capillary column, using an electrokinetic system of phosphate buffer 5mmol/L (Na_2HPO_4) and SDS 50mmol/L (pH=9.06).