附马风湿片中士的宁碱和马钱子碱的含量测定

Study on the Quantity of Strychnine and Brucine from Fumafengshi Tablets

采用双波长薄层扫描法测定附马风湿片中士的宁碱和马钱子碱的含量。士的宁碱和马钱子碱的最大吸收波长分别是为260 nm和304 nm,参比波长均为350 nm。本法使两组分在同一薄层板上完成定量分析。操作简便,结果满意,为有效控制产品质量提供了依据。

This paper reported the determination of strychnine and Brucine from Fumafengshi tablets by the dualwavelength thin layer chromatography scanning method. The sample wavelenthes of the two compounds are 260nm and 304nm, respectively, the reference wavelength being the same as 350nm, Sx=3, the slit being 1.2mm×1.2mm, and the standard curve being linear within 1.002-5.01μg and 0.525-2.625μg. The correlation coefficient is 0.9991 and 0.9997, the average recovery is 97.7%(n=4) and 100. 72%(n=5), and the CV% of this method is 4.46%(n=6) and 0.71%(n=6), respectively. This method is very special, rapid, simple and accurate. It is suitable for quality control of Fumafengshi tablet.