核糖核酸抗大鼠肝纤维化的作用

Effects of RNA on anti-hepatic-fibrosis in rat

用二甲基亚硝胺(DMN)诱发大鼠肝纤维化,分别以RNA(4 mg·kg^(-1))和辅酶Q_(10)(2.5 mg·kg^(-1))治疗,并设正常和病理对照组。治疗后30,60和90 d杀鼠,作血清酶学、氨基酸检测、胶原纤维定量及电镜检查.结果表明RNA和辅酶Q_(10)治疗组ASTγ—GT均在正常范围,A/G比值与病理对照组同期比较,差异显著。RNA组ALT、MAO和NAG明显下降,血清氨基酸水平升幅较小,光镜和电镜检查显示RNA治疗组肝细胞变性坏死及肝纤维化程度都比较轻,提示RNA有降酶保肝和抗肝纤维化作用。

Hepatic fibrosis in rat induced by intraperitoneal injection of dimethylnitroamine (DMN) was traeted by RNA (4 mg · kg-1) and CoQ10(2. 5 mg · kg-1) ,seperately. Normal and pathological groups were compared. In 30, 60 and 90 days treatment, rats were killed for ul-trastructural examination and measurement of serum enzymology, serum amino acids and the quantity of collagenous fibers. The results indicated that, in RNA-treated and CoQ10 treated groups, Aspartate aminotransferase (AST) and Y-glutamyl-transpeptidase(γ-GT) were normal, the ratio of albunm to globulin (A/G) had significantly difference compared with control group. In RNA-treated group,L-Alanine:2-Ox-oglutarate Aminotransferase(ALT), monoamine oxidase (MAO) and N-acetyl-β-glucosaminidase (NAG) decreased evidently, serum amino acids increased slightly. Ultrastructural and microscopical examination showed the degree of hepa-tocytic necrosis degeneration and the quantity of collagenous fibers in RNA-treated group were rather mild. It appeared that there were protection effects of RNA on liver enzymology and hepaticfibrosis.