人工虫草碱提取物的降血糖作用研究

Study on Hypoglycemic Activity of Alkaline Extract from cultural Mycelium of Cordyceps sinensis

人工虫草菌丝的碱(1.25 mol·L^(-1)NaOH)提醇沉淀物CsOH(产率2.58％)经纸层析(PPC)和气相层析分析证实为含5种单糖的杂多糖。口服给予CsOH在剂量500、250mg·kg^(-1)时和腹腔给予CsOH在剂量100、50 mg·kg^(-1)时对正常小鼠、四氧嘧啶糖尿病模型小鼠和链脲佐菌素糖尿病模型小鼠均有显著的降血糖作用.且呈现一定的量效关系。

The dried powder of cultural mycelium of ordyceps sinensis was extracted with 1.25 mol · L-1sodium hydroxide and precipitated with ethanol to afford the CsOH, in 2.58% yield. CsOH was consisted of 5 monosaccharide measured by paper chromatogra-phy (PPC) and gas- liquid chromatography (GLC). CsOH given intragastrically at the dose of 500 mg·kg-1and 250 mg·kg-1,ip at a dose of 100 mg·kg-1and 50 mg·kg-1had significant hypoglycemic activities in normal mice, alloxan- induced diabetes mice and streptozotocin-induced diabetes mice, and showed a dose-effect relation.