一氧化氮对妊娠高血压综合征发病的影响

Role of Nitric Oxide in pathogenesis of pregnancy induced hypertension

目的 :研究一氧化氮 (NO)对妊高征发病的影响。方法 :用一氧化氮合酶 (NOS)抑制剂L 硝基 精氨酸甲酯 (L NAME)抑制孕鼠体内NO合成 ,观察血压、尿蛋白、子鼠体重、血NO、血内皮素 (ET 1)水平变化 ;同时观察NO供体硝酸甘油释放NO ,对L NAME作用的影响。结果 :模型组孕鼠较对照组血压升高 ,尿蛋白增多 ,子鼠体重下降 ,血NO水平下降 ,血ET 1水平上升 (均 P<0 0 1) ;治疗组孕鼠较模型组血压下降 ,尿蛋白减少 ,子鼠体重增加 ,血NO水平上升 ,血ET 1水平下降 (均P <0 0 1)。结论 :NO水平下降是妊高征发病的因素之一。

Objective: We attempted to investigate the role of nitric oxide(NO) in pathogenesis of pregnancy induced hypertension(PIH). Methods: No nitro L arginine methyl ester(L NAME) was used to inhibit nitric oxide synthase(NOS) in pregnant rats. Indices including blood pressure, urine protein concentration, pup weight, serum NO level and plasma ET 1 level were measured. Nitroglycerin, a nitric oxide donor, was used to observe its impact on the effect of L NAME at the same time. Results: Infusion of L NAME elevated blood pressure, increased urine protein concentration, decreased pup weight, decreased serum NO level and raised plasma ET 1 level(P<0.01, respectively); However in the L NAME treated animals nitrolycerin significantly lowered blood pressure, decreased urine protein concentration, increased pup weight, increased serum NO level and decreased plasma ET 1 level(P<0.01, respectively). Conclusions: ① Infusion of L NAME, an inhibitor of NOS, causes decreased serum NO level and some signs similiar to preeclampsia such as hypertension, proteinuria and intrauterine growth retardation(IUGR). Nitroglycerin can reverse the lesions induced by the treatment of L NAME. These findings indicte that reduced level of NO may be a factor responsible for PIH. ② NO can decrease the plasma ET 1 level. NO may regulate the process of PIH via ET 1 pathway. It may be one mechanism for NO. ③ An useful animal model for PIH is provided to test movel therapeutic and preventive stratages. [