热休克预处理诱导HSP70蛋白表达减轻神经细胞的缺血损伤

Heat shock protein 70 expression following transient ischemia in the gerbil

目的 :研究热休克预处理对脑缺血再灌后热休克蛋白 70 (HSP70 )的表达影响及对缺血神经细胞损伤的保护作用。方法 :采用沙鼠短暂前脑缺血再灌损伤模型 ,光镜观察缺血再灌后神经细胞损伤情况 ,免疫组化方法检测脑缺血后不同时期额叶HSP70蛋白表达。结果 :沙鼠脑缺血后HSP70蛋白仅在再灌后 1d有少量表达 (P <0 .0 1) ;缺血神经细胞在再灌后第 7d大多出现损伤改变 ;热休克预处理能增加沙鼠脑缺血再灌后各期HSP70蛋白表达 (P <0 .0 1) ,明显减轻缺血神经细胞损伤 (P <0 .0 1)。结论 :热休克预处理能通过增加HSP70蛋白表达 ,减少缺血神经细胞损伤。

Objective: To study the expression of heat shock protein 70(HSP70) following transient ischemia in the gerbil. Methods: Immunohistochemistry(IHC) was used to determine the expression of the HSP70 protein in the forebrain during different periods after post ischemic reperfusion in the gerbil, and the neuronal damage was examined with microscope. Results: The HSP70 protein was only expressed at the 1st day of reperfusion(P<0.01); and most of the neurons showed damage on the 7th day of reperfusion; heat preconditioning could increase the expression of HSP70 protein(P<0.01) and decrease the neuronal damage(P<0.001) after 6 min of ischemia. Conclusion: Heat preconditioning could protect neural cells from damage by increasing the expression of the HSP70 protein. [