摘要
激光共聚焦显微镜 (LSCM)是九十年代初出现的一种可用荧光标记进行微形态观察的高分辨显微镜 ,与传统方法比较 ,具有精确、清晰、动态和三维成像等突出优点。骨计量学是研究代谢性骨病的重要方法。本研究将激光共聚焦显微技术引入骨计量学中 ,达到了精确定位和定量分析骨微结构的目的 ,具有无须染色 ,对厚片可进行荧光染色观察的特点 ,其清晰度高 ,制片易 ,可与组织化学、免疫组织化学等方法结合 ,从多个角度、多个层次同时观察骨微结构与各种骨细胞的形态与功能。为代谢性骨病的研究和诊断提供了新的方法和途径。
The laser scanning confocal microscope(LSCM) is the new high distinguishing microscope, which can be used in observation on fluorescence, since 1990. The bone morphometry is an important way to study metabolic bone diseases. Our study on research combination of LSCM with bone morphometry in observing microarchitecture of bone found that LSCM could make the photo of bone trabecula distinctly and its location accurately. Because of its technical advantages, LSCM could link histochemical or immunohistochemical method for the bone morphometry and bone cells at the same time and obserrate the thick slide. It is a new method and way to study and diagnose metabolic bone diseases. [
出处
《湖南医科大学学报》
CSCD
2000年第4期413-415,共3页
Bulletin of Hunan Medical University
基金
国家自然科学基金!资助 (编号 :3 9970 3 4 7)
