凝血酶对牛主动脉内皮细胞表达组织因子活性的影响

Effects and mechanism of thrombin on the expression of tissue factor activity in cultured bovine aortic endothelial cells

目的 :探讨凝血酶对内皮细胞表达组织因子 (TF)活性的作用及其机制。方法 :实验材料为第 4～ 8代新生牛主动脉内皮细胞 ,采用一期凝固法检测细胞冻融液中的TF活性。结果及讨论 :凝血酶可促进血管内皮细胞表达TF活性 ;凝血酶对内皮细胞的这种刺激作用可能具有Ca2 + /CaM依赖性 ,且可能与细胞内cAMP浓度有关。

Objective: To explore the effects and mechanism of thrombin on the expression of tissue factor(TF) activity in cultured bovine aortic endothelial cells(BAECs). Methods: BAECs were cultured according to Schwartz's method, and one stage clotting time assay was used to measure TF activity. Results: ①Thrombin enhanced the expression of TF activity in cultured BAECs. ②A 23187 , a calcium ionophore, induced slightly the expression of TF activity, but markedly promoted the thrombin induced TF activity in BAECs(P<0.05). ③TFP, a Ca 2+ /CaM inhibitor, inhibited the inductive effects of thrombin. ④Pentoxifylline, a phosphodiesterase inhibitor, inhibited thrombin induced TF activity in BAECs. Conclusion: Thrombin can induce the expression of TF activity in cultured BAECs, and the processes of signal transduction may be associated with Ca 2+ /CaM dependent pathway and cAMP. [