鼠血清中TNF活性及HGF、PGE_1保护肝脏作用的研究

Study on Changes of Tumor Necrosis Factor Activity and Protective Effect of Hepatocyte Growth Factor and Prostaglandin E_1 to Liver

本试验应用氨基半乳糖及内毒素,致大白鼠实验性肝坏死,应用肝细胞生长素(HGF)或前列腺素E_1(PGE_1)观察对肝细胞的保护作用,以结晶紫染色法检测鼠血清中肿瘤坏死因子(TNF)活性。结果表明,实验性肝坏死时,血清中TNF活性较对照组明显升高,HGF及PGE_1能保护肝脏,并降低血清中TNF活性。作者认为,TNF与实验性肝坏死发病机制有关,HGF及PGE_1能抑制内毒素诱导的TNF产生,从而防止肝坏死。

Galactosamine and lipopolysaccharide (LPS) were used to cause experimental liver necrosis in rats. Hepatocyte growth factor (HGF) and prostaglandin E_1 (PGE_1) were used to protect the experimental liver. Serum tumor necrosis factor (TNF) activity was detected by crystal violetstaining method. A significant elevation of TNF activity was observed in the experimental liver necrosis group but not in the control. HGF and PGE_1 protected the liver and decreased serum TNF activity in rats. The paper suggests that serum TNF may play an important role in the pathogenesis of experimental liver necrosis, that HGF and PGE_1 seem to be a protection of in liver injury by decreasing serum TNF activity.