摘要
使用a_1(Ⅰ)、a_2(Ⅰ)及a_1(Ⅲ)人胶原cDNA探针,采用斑点杂交技术观察石英粉尘对体外培养的人胚肺成纤维细胞胶原mRNA合成的影响。于培养的成纤维细胞培养液中加入不同剂量(100、200、500及1000μg/10ml)的石英粉尘,第1天后a_1(Ⅰ)、a_2(Ⅰ)及a_1(Ⅲ)胶原mRNA水平即开始升高;第3、5、7、9天,mRNA水平均高于同期不加尘的正常对照组。在同样的加尘剂量下,加尘后第1天a_1(Ⅲ)mRNA的增加明显高于a_1(Ⅰ)及a_2(Ⅰ)胶原mRNA的增加。本研究证明石英粉尘促进成纤维细胞胶原mRNA合成,提示在矽肺病进程中,胶原转录增强是纤维化的关键步骤。
Human a_1(Ⅰ), a_2(Ⅰ) and a_1(Ⅲ) cDNA probes and RNA dot hybridization were employed to quantitate collagen mRNA changes after adding silica dust into the media of human 2BS fibroblasts. At all dosages used (100, 200, 500, and 1000μg), the a_1(Ⅰ), a_2(Ⅰ) and a_1(Ⅲ) mRNA levels increased one day after dusting. At the same dosage of silica (100μg), a_1(Ⅲ) mRNA increased more rapidly than did type Ⅰ collagen mRNA. The type Ⅰ and type Ⅲ collagen mRNA contents in the experimental groups were higher than those in controls on days 3, 5, 7 and 9.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1993年第1期1-5,共5页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
