抗猪游离E受体McAb的研究

Study on Monoclonal Antibodies Against Porcine Serum E Receptor

人、猪E受体(即CD_2)有共同抗原性,E受体具有膜结合型及游离型两种形式.人血清中游离E受体的水平,与初生、老年、妊娠、头颈部肿瘤和免疫性疾病相关。该分子从细胞表面脱落后,可以再生,提示它参与T细胞的活化与增殖反应。这些结果与1984年Meuer等提出CD_2介导不依赖于特异抗原T细胞激活的一个旁路相一致。CD_2还能增加T细胞和抗原提呈细胞或靶细胞的粘附性和可塑性,藉其体内的天然配体LFA-3与多种细胞发生反应,在机体免疫调节中起重要作用. 为进一步探讨CD_2结构与功能,本实验应用细胞融合技术,建立了三株能持续分泌抗猪游离E受体McAb的杂交瘤细胞株,并对其性质进行了研究。

Three hybridoma cell lines producing monoclonal antibodies (McAbs) against porcine serum E receptor (PSER) were established by fusing mouse myeloma SP2/o cells with spleen cells of BALB/c mouse immunized with PSER.The ascites McAbs were purified by ammonium sulfate precipitation and DEAE-cellulose chromatography. The McAbs were found to belong to the IgGl and IgG2b subclasses, respectively. These McAbs inhibited reverse E-rosette formation and enhanced or depressed lymphocyte proliferation induced by PHA or ConA. The specificity of these McAbs was proved by immuno-dot blot and Western blot assay.The potential application of these McAbs in the study of structure and biological function of the E-receptor is discussed.