大鼠睾丸支持细胞内中间丝分布的电镜研究

Distribution of the Intermediate Filaments in Rat Sertoli Cells:An Electron Microscopic Study

用透射电镜观察大鼠睾丸支持细胞内中间丝(IFs)的分布结果表明:IFs主要集中于细胞核周围,杂乱排列成网,并自核周区向周围胞质内广泛伸展,联系多种细胞器,并与邻近支持细胞细胞膜处桥粒样结构、外质特化结构,与邻近生精细胞细胞膜处半桥粒样结构等相联系。提示支持细胞内IFs是以核周为中心,联系胞质、胞膜多种结构的精细网络系统。

The distribution of intermediate filaments (IFs) in the Sertoli cells of rat testis was studied under a transmission electron microscope. The results showed that IFs in Sertoli cells were centrally arranged around the nucleus in a crisscross manner.From the perinuclear region the IFs had widespread extensions and were connected with the following structures: 1) with the mitochondria, Golgi apparatus, endoplasmic reticulum and other subcellular structures; 2) with the desmosome-like and hemi-desmosome-like junctions with adjacent Sertoli and spermatogenic cells; 3) with the microfilaments (MFs) in the ectoplasmic specialization (ES) surrounding the head of elongated stage Ⅰ-Ⅴ; sperma-tids; 4) with the MFs in ES between adjacent Sertoli cells. In conclusion, IFs form a delicate intracellular network in Sertoli cell with its center in the perinuclear region and stretching and connecting with many subcelluclar structures in the cytoplasm and cell membrane.