不同旋光异构体棉酚对睾丸支持细胞的影响

The Effects of Optical Isomers of Gossypol on Sertoli Cells In Vitro

研究不同旋光异构体棉酚对体外培养大鼠睾丸中支持细胞的影响。观察到不同旋光异构体棉酚均能引起支持细胞形态产生变化,细胞器中以线粒体变化最明显,表现为SDH活性减弱,有些酶粒变粗,电镜下看到线粒体肿胀,嵴排列不规则,甚至断裂或消失。细胞受损伤程度按(+)<(±)<(-)棉酚程序。上述变化与口服棉酚大鼠睾丸中支持细胞变化相似。

This study was designed to investigate the effects of gossypol optical isome-rs on Sertoli cells of 20-day-old Wistar rats in vitro. The dosages used were 10μg/ml of (±) and (+) gossypol and 5μg/ml (-) gossypol. After 24 h in culture, we found that different optical isomers induced different degrees of morphological changes in Sertoli cells. The changes in mitochondria were obvious. It was shown that the activity of SDH in mitochondria was reduced, or some enzymatic granules became coarser, after gossypol treatment. Under EM we found that the mitochondria had become swollen, the cristae were arranged irregularly, or even broken or absent. The damage caused by the different optical isomers on Sertoli cells was in the order of(+)<(±)<(-) gossypol. These changes coincided with changes in Sertoli cells of rats following oral administration of gossypol.