细菌脂多糖对骨髓瘤细胞胞浆游离钙的影响

Effect of Lipopolysaccharide on Cytosolic Free Calcium Concentration in Myeloma Cells

以钙离子荧光指示剂Quin 2测定胞浆游离钙,研究细菌脂多糖对骨髓瘤细胞胞浆游离钙浓度的影响。骨髓瘤细胞与脂多糖孵育后,胞浆游离钙浓度显著增高,植物血凝素升高胞浆游离钙浓度的作用受到明显抑制。十四脂酰佛波乙酸酯对骨髓瘤细胞胞浆游离钙浓度无影响,但对植物血凝素升高胞浆游离钙浓度的作用有明显抑制。提示脂多糖引起细胞钙平衡失调,并干扰细胞钙信号的产生。

The concentration of cytosolic free calcium was measured using the fluorescent Ca2+-indicator quin 2, and the effect of lipopolysaccharide (LPS) on cytosolic free calcium in myeloma cells was studied. Incubation of myeloma cells with LPS elicited a significant increase in cytosolic free calcium, and inhibited the phytohaemagglutinin (PHA) -induced elevation in cytosolic free calcium. Treatment of the cells with phorbol-12-myristate-13-acetate (PMA) failed to affect the concentration of cytosolic free calcium in resting cells, but inhibited the subsequent PHA-induced elevation in cytosolic free calcium. It is suggested that LPS causes alterations in intracellular calcium homeostasis and perturbations in the calcium-dependent signal transduction pathway.