白念珠菌特异DNA片段与核酸杂交研究

Investigation of a Candida albicans-specific DNA Fragment Used for Nucleic Acid Hybridization

用EcoRI对白念珠菌标准株Cli DNA做酶切分析,获得2.5kb片段,经^(32)P标记后分别与各种念珠菌、隐球菌和细菌进行核酸杂交。结果该片段与所有白念珠菌杂交强阳性,与近平滑念珠菌和1株热带念珠菌出现微弱杂交信号,与其它菌无杂交。提示该片段含有白念珠菌种特异性碱基序列,可望进一步制备成白念珠菌基因探针。

A 2.5 kb fragment from Candida albicans Cli EcoR I-digested was labeled with 32P and used to probe blots of DNA from various organisms. DNA from all strains of C. albicans gave evidence of strong sequence homo-logy with the 2.5 kb fragment. With the exception of weak hybridization signals from strains of C. parapsilosis and a strain of C. tropicalis, DNA from other Candida species, Cryptococcus as well as bacteria did not hybridize with the probe.The results indicate that the 2.5 kb fragment might contain sequence unique to C. albicans. After this is confirmed by cloning, a specific probe for the identification of C. albicans will be made.