斑点免疫结合法检测三种类型的类风湿因子及其临床意义

Dot Immunobinding Assay for Detection of Three Types of Rheumatoid Factor and Its Clinical Significance

应用斑点免疫结合法技术建立了检测IgM、IgG和IgA 3种类型类风湿因子的方法,并对86例类风湿关节炎患者血清中这三种类风湿因子进行了检验分析。IgM、IgG和IgA类风湿因子的阳性率分别为59.9%、54.7%及17.4%,总阳性率为72.8%,同时采用乳胶凝集法检测阳性率为43.0%。结果表明,斑点免疫结合法明显优于乳胶凝集法。在同一患者血清中,具有IgM及IgG类风湿因子两项阳性时,或具有IgM、IgG及IgA 3项类风湿因子阳性时,常反应病情重,出现关节外症状较多见。提示同时检测3种类型的类风湿因子有助于对病情判断,这也是斑点免疫结合法测定类风湿因子的一个优点。

We set up a dot immunobinding assay method for determination of IgM-, IgG- and IgA-rheatoil factor (RF) and report here the results in 86 patients with rheumatoid arthritis (RA).It was found that the positive rates of IgM-,IgG-and IgA-RF were 59.7%,54.7% and 17.4% respectively.The total positive rate was 72.8%.The positive rate using the Latex method was only 43.0%.Our results reveal that dot immunobinding assay for RF is more sensitive than the Latex method.Furthermore,there was a profound correlation of IgG-RF with active stage and the frequency of extra-artivular manifestations in RA patients.