小鼠肺腺癌LA795细胞系具不同转移潜能亚系的体内侵袭力及定量分析

Comparative Study and Morphometrical Analysis of the In Vivo Invasiveness of Mouse Lung Adenocarcinoma Variants with Different Metastatic Potentenials

利用肾包膜下体内侵袭模型,对小鼠肺腺癌具不同转移潜能亚系LA1、LAD和LA5的体内侵袭能力进行比较观察。结果表明:3个亚系的体内侵袭均呈进行性,瘤组织侵袭生长区面积不断增大,所占总面积的百分比不断升高。其中转移潜能较强的LA5亚系侵袭程度、瘤组织所占面积的百分比均较高,显示了较强的体内侵袭能力;转移潜能较弱的LAD居中;不转移的LA1亚系瘤组织侵袭程度、所占面积的百分比均较低。3个亚系的体内侵袭能力与其转移潜能相一致.

The in vivo invasiveness of LA1, LAD and LA5 variants of mouse lung adenocarcinoma (LA795) with different metastatic potentials was studied comparatively.The in vivo invasive abilities of LA1, LAD and LA5 were surveyed after subrenal capsule inoculation of a 1-nm3 tumor fragment of the respective variant. The morphometrical study included measurement of the whole tissue section area (TA), tumor tissue area (SA) and SA/TA (indicating tumor invasion ability). The results of histological observation were in accordance with those of morphometrical measurement. The invasion was found to be progressive, with a progressively enlarging tumor invasion area and an increasing SA/TA ratio in all cases. The invasiveness of LA1, LAD and LA5 in vivo was correlated with metastatic potential.