摘要
成年大鼠永久性结扎右侧颈总动脉,烧凝右侧大脑中动脉并暂时夹闭左侧颈总动脉,造成右侧皮层局灶性缺血模型。采用电子自旋共振(ESR)技术,测定右侧皮层组织的自由基水平;同时用比色法测定丙二醛(MDA)含量。发现动物缺血后12h右侧皮层的自由基和MDA含量均明显升高;而术中一次注射混合型神经节苷脂(GM,50mg/kg,ip)的动物,脑缺血后12h,自由基和MDA含量与正常对照动物比较无明显差别。阻断脑血流可导致皮层自由基增加,伴有自由基诱发的脂质过氧化产物MDA增多。GM通过抑制缺血皮层自由基的生成,减轻脂质过氧化程度,对缺血脑组织起到一定的保护作用。
The animal model of focal ischemic stroke was prepared in adult rats by permanent ligation of right common carotid artery ( CCA) and middle cerebral artery (MCA), and clamping of the left CCA for 1 hour. The levels of free radical and malondialdehyde( MDA) were measured by electron spin resonance(ESR) spectroscopy and photometry, respectively. 12 hours after the operation, the levels of free radical and MDA of right cortex in ischemia group were significantly higher than those in normal control, while in mixed gangliosides (GM) -treated ischemia group, both parameters restored to normal control levels. The results showed that focal cortical ischemia induced increase in free radicals and MDA, which could be inhibited by one dose(50μg/ kg) of GM.
出处
《中国应用生理学杂志》
CAS
CSCD
1993年第4期311-313,共3页
Chinese Journal of Applied Physiology
基金
国家自然科学基金
广东省科学基金
关键词
脑缺血
自由基
丙二醛
神经节苷脂
cerebral ischemia
free radical
electron spin resonance
malondialdehyde
gangliosides
